Abstract:
Positive plant selectable marker genes are commonly used in plant transformation because they not only enhance
the frequency of generation transgenic tissues but are considered biosafe, unlike antibiotic or herbicide resistance
genes. In this study, the binary vector pNOV2819-ubiamiRNA3PARP1, harbouring the phosphomannose
isomerase (pmi) gene was developed and used in recovery of transgenic maize (Zea mays L.) plants containing the
drought tolerance gene, amiRNA3-PARP1. The pre-amiRNA3-PARP1 and Tnos transgenes were sequentially
PCR-cloned upstream the ubiquitine promoter in the Ubi/NC1300 plasmid. The pre-amiRNA3-PARP1 expression
cassette was transferred into the pmi gene-containing pNOV2819 plasmid to produce the pNOV2819-
ubiamiRNA3PARP1 vector. Transgenic IL3 and A188 plants containing pre-amiRNA3-PARP1 were generated
through transformation with LBA4404 harbouring the pNOV2819-ubiamiRNA3PARP1 vector. The plants were
confirmed transgenic by PCR. It is clear that the developed vectors are effective in recovery of amiRNA3-
PARP1 transgenic tissues and plants containing the pmi gene, which has been shown to have no negative
environmental or health effects.
